Technical Data
B0003-05T1
Bacillus anthracis PA (Protective Antigen) (Anthrax)
Description:
After inhalation by mammals, Bacillus anthracis spores germinate in alveolar macrophages then migrate to lymph nodes where they multiply. The vegetative bacteria excrete the tripartite exotoxin which consists of three polypeptides: protective antigen (PA, 83kD), lethal factor (LF, 90kD) and oedma factor (OF, 89kD). The two components (OF and LF) of the toxin enzymatically modify substrates within the cytosol of the mammalian cells: The OF is an adenylate cyclase that impairs the host defenses through a variety of mechanisms inhibiting phagocytosis. The LF is a zinc dependent protease that cleaves several mitogen activated protein kinase kinases (MAPKK) and causes lysis of macrophages. To intoxicate mammalian cells, the third component of the toxin PA, binds to a ubiquitously expressed cellular receptor, Tumor Endothelium Marker-8 (TEM8). Upon binding to TEM8, PA is cleaved into 20 and 63kDa fragments (PA20 and PA63) by furin or furin-like proteases. PA20 dissociates into medium and allows the PA63 fragment to heptamerize and bind LF and OF of the toxin. The resulting complex of PA63 fragment with EF and/or OF binds to PA-receptor TEM8/ATR and internalized into endosomes followed by translocation of LF and OF into cytosol of the cells.

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-10ug/ml. Using ECL technique.
ELISA: 0.1-1ug/ml; Use B0003-05T5 or B0003-05T6 to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher.

Matched Pairs:
Capture: B0003-05T
Detection: B0003-05T1

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG17C16Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceMouse
Concentration:
As reported
Immunogen:
B. anthracis Protective Antigen (PA)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity:
Recognizes Bacillus anthracis Protective antigen (Anthrax). Does not crossreact with Lethal Factor (LF) of Bacillus anthracis, Y. Pestis, F. Tularensis and Toxoplasma gondi. The crossreactivity in various species is not known.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Bradley KA et al (2001) Nature 414, 225-229; liu S and Leppla SH (2002) JBC (in press); Leppla, SH (1982) PNAS 79, 3182; OBrien J et al (1985) Infect Immun 47, 306; Duesbery, NS et al (1998) Science 280, 734