Technical Data
B0003-63P
BAF57 (BRG1 Associated Factor 57, Mammalin Chromatin Remodeling Complex, SMARCE1, SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin e1)
Description:
The SWI/SNF complex in S. cerevisiae and Drosophila is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. The complex contains an ATP-dependent nucleosome disruption activity that can lead to enhanced binding of transcription factors. The BRG1/brm associated factors, or BAF, complex in mammals is functionally related to SWI/SNF and consists of 9 to 12 subunits, some of which are homologous to SWI/SNF. A 57kD BAF subunit, BAF57, is present in higher eukaryotes, but not in yeast.

Cellular Localization: Nuclear

Positive Control: HeLa or Jurkat whole cell lysate.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:16,000.
Western Blot: 1ug/ml. ~ 37kD band in Hela and Human Brain lysates.
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide: PPTDPIPEDEKKE, representing the C-terminus of the human protein (residues 399-411), according to NP_003070.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.
Specificity:
Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse (99% identity with immunogen) due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Wang W et al. Architectural DNA binding by a high-mobility-group/kinesin-like subunit in mammalian SWI/SNF-related complexes. Proc Natl Acad Sci U S A 95:492-8 (1998).