Technical Data
B0003-63P1
BAF57 (BRG1 Associated Factor 57, Mammalin Chromatin Remodeling Complex, SMARCE1, SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin e1)
Description:
The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to amino acid residues 360 and the C-terminus(residue 411) of human BRG1-Associated Factor 57 (SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator of Chromatin E1) using the numbering given in entry NP_003070.3 (Gen
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Specificity:
Species Crossreactivity: Human. Mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.