		Technical Data
B0003-64D1	BAFF (B cell Activating Factor belonging to TNF Family, BLyS, TALL-1, THANK) (FITC)

Description:
Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF, BLyS, TALL-1, THANK, and zTNF4. BAFF/BLyS was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. Two receptors, TACI and BCMA, for BAFF were originally identified. A third receptor was identified recently and designated BAFF-R and BR3 for BLyS receptor 3. Unlike BCMA and TACI, which bind to BAFF and April, BAFF-R/BR3 is specific for BAFF and plays a predominant role in BAFF induced B cell development and survival. BAFF and its receptors are involved in B cell associated autoimmune diseases, and activate NF-kB and c-jun N-terminal kinase.

Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen and Paraffin sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgM	6D7	Highly Purified

Size		Storage	Shipping	Source	Host
100 Tests		-20°C	Blue Ice	Human	Rat

Concentration:

~1mg/ml

Immunogen:

Recombinant human soluble BAFF (aa83-285).

Purity:

Purified by chromatography. (>95% SDS-PAGE).

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

Specificity:

Recognizes membrane-bound human and mouse (weak) BAFF.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. J. Groom, et al.; J. Clin. Invest. 109, 59 (2002) 2.A. J. Novak, et al.; Blood 103, 689 (2004) 3. B. He, et al.; J. Immunol. 172, 3268 (2004) 4. B. Huard, et al.; Int. Immunol. 16, 467 (2004) 5. A.J. Novak, et al.; Blood 104, 2247 (2004) 6. M.V. Jonsson, et al.; J. Clin. Immunol. 25, 189 (2005) 7. F. Mackay, et al.; J. Exp. Med. 190, 1697 (1999) 8. P.A. Moore, et al.; Science 285, 260 (1999) 9. A. Mukhopadhyay, et al.; J. Biol. Chem. 274, 15978 (1999) 10. B P. Schneider, et al.; J. Exp. Med. 189, 1747 (1999) 11. H.B. Shu, et al.; J. Leukoc. Biol. 65, 680 (1999) 12. Severe B cell hyperplasia and autoimmune disease in TALL-1 transgenic mice: S.D. Khare, et al.; PNAS 97, 3370 (2000)