Technical Data
B0003-64H
BAFF (B cell Activating Factor belonging to TNF Family, BLyS, TALL-1, THANK)
Description:
Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF, BLyS, TALL-1, THANK, and zTNF4. BAFF/BLyS was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. Two receptors, TACI and BCMA, for BAFF were originally identified. A third receptor was identified recently and designated BAFF-R and BR3 for BLyS receptor 3. Unlike BCMA and TACI, which bind to BAFF and April, BAFF-R/BR3 is specific for BAFF and plays a predominant role in BAFF induced B cell development and survival. BAFF and its receptors are involved in B cell associated autoimmune diseases, and activate NF-eB and c-jun N-terminal kinase.

Applications:
Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilution to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG16D144Highly Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Extracellular domain of human BAFF (aa 137-285).
Purity:
Purified from concentrated hybridoma tissue culture supernatant. 95% (SDS-PAGE).
Form
Supplied as a liquid in PBS containing 0.02% sodium azide.
Specificity:
Recognizes C-terminus but not the stalk of human BAFF. Does not cross-react with mouse BAFF
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
BAFF and APRIL: A Tutorial on B Cell Survival: F. Mackay, et al.; Annu. Rev. Immunol. 21, 231 (2003).