Technical Data
B0003-64K2
BAFF (B cell Activating Factor belonging to TNF Family, BLyS, TALL-1, THANK) (FITC)
Description:
Applications:
Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1ul stains 200,000 cells in 50ul sample volume
Flow Cytometry: 0.5-5ug/ml per 5x10e5 cells. Tested using 293T cells expressing suface BAFF vector or mock transfected. Recommended FACS buffer: PBS, 5% FCS, 0.02% sodium azide.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.


TypeIsotypeCloneGrade
MabIgG2a6D8Highly Purified
SizeStorageShippingSourceHost
100Tests4C (-20C Glycerol)Blue IceHumanRat
Concentration:
~1mg/ml
Immunogen:
Recombinant human soluble BAFF (aa 83-285).
Purity:
Highly purified (SDS-PAGE).
Form
Supplied as a liquid in PBS containing 0.02% sodium azide. Labeled with FITC.
Specificity:
Recognizes the stalk region present in membrane-bound human BAFF. Detects endogenous protein by Flow Cytometry
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Product Specific Literature References
1. BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth: P. Schneider, et al.; J. Exp. Med. 189, 1747 (1999)
2. BAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population: J.S. Thompson, et al.; J. Exp. Med. 192, 129 (2000)
3. A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth: P. Rennert, et al.; J. Exp. Med. 192, 1677 (2000)
4. BAFF and APRIL protect myeloma cells from apoptosis induced by interleukin 6 deprivation and dexamethasone: J. Moreaux, et al.; Blood 103, 3148 (2004)

GENERAL LITERATURE REFERENCES
1. BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth: P. Schneider, et al.; J. Exp. Med. 189, 1747 (1999)
2. BAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population: J.S. Thompson, et al.; J. Exp. Med. 192, 129 (2000)