Technical Data
BAFF, Recombinant, Human (B cell Activating Factor belonging to TNF Family, BLyS, TALL-1, THANK)
Molecular Biology Storage: -20CShipping: Blue Ice
Human BAFF (for B cell activating factor belonging to the TNF family) is a newly discovered novel ligand of the TNF family. BAFF plays an important role as costimulator of B cell proliferation and function. Recombinant Human BAFF is a soluble 17kD protein containing 153 amino acid residues, comprising the TNF-like extra-celluar domain of BAFF.

Biological Activity:
Assay#1: Determined by the dose-dependent stimulation of IL-8 production by Human PBMC (Peripheral Blood Mononuclear Cells). The ED50 for this effect is 5-10ng/ml. Please Note: Results may vary with PBMC donors.
Assay#2: Determined by a mouse splenocyte survival assay. The expected ED50 for this effect is 0.5-2.0ug/ml.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin: USA
Source: E. coli
Purity: 95% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug).
Form: Supplied as a sterile filtered, lyophilized powder from PBS, pH 7.5, 0.5 DTT. Reconstitute in dH2O to a concentration of 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.