Technical Data
B0003-66A
BAFF, Recombinant, Human (B cell Activating Factor belonging to TNF Family, BLyS, TALL-1, THANK)
5ug
20ug
Molecular Biology Storage: -20CShipping: RT
The TNF-related ligand, B cell-activating factor belonging to the TNF family (BAFF), is necessary for normal B cell development and survival, and specifically binds the receptors transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI), B cell maturation Ag (BCMA), and BAFF-R.Using specific mAbs, BAFF-R was found to be the predominant BAFF receptor expressed on peripheral B cells, in both humans and mice, and antagonist mAbs to BAFF-R blocked BAFF-mediated costimulation of anti- micro responses. The other BAFF receptors showed a much more restricted expression pattern, suggestive of specialized roles.

Recombinant Human B Lymphocyte Stimulator is a single, non-glycosylated polypeptide chain containing 153 amino acids and having a molecular mass of 17,007 Daltons.

Source:
E. coli

Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Val-Gln-Gly.

Biological Activity:
Recombinant B Lymphocyte Stimulator is fully biologically active when compared to standard. The activity is determined by the dose-dependent stimulation of IL-8 production by human PBMC. The ED50 for this effect is less than 10ng/ml. Please note: results may vary with PBMC donors.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280.
2. Analysis by RP-HPLC, using a standard solution of BAFF as a Reference Standard.

Reconstitution:
Reconstitute the lyophilized BAFF in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder in mannitol, HSA.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Fu C, Tian L, Kan B, Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2004 Dec;21(6):897-900.
2. Morrison MD, Reiley W, Sun SC, J Biol Chem 2005 Jan 11.
3. Krumbholz M, Theil D, Rosenwald A, Hess DM, J Exp Med 2005 Jan 10.
4. Laird MW, Sampey GC, Zukauskas D, Li Y, Protein Expr Purif 2005 Feb;39(2):237-46.
5. Gottenberg JE, Busson M, Lavie F, Mariette X, Ann Rheum Dis 2005 Jan 7.
6. Castigli E, Wilson SA, Dedeoglu F, Jabara H, J Exp Med 2005 Jan 3;201(1):35-9.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.