Technical Data
BAFF, Recombinant, Human (B cell Activating Factor belonging to TNF Family, BLyS, TALL-1, THANK)
Molecular Biology Storage: -20CShipping: RT
The TNF-related ligand, B cell-activating factor belonging to the TNF family (BAFF), is necessary for normal B cell development and survival, and specifically binds the receptors transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI), B cell maturation Ag (BCMA), and BAFF-R.Using specific mAbs, BAFF-R was found to be the predominant BAFF receptor expressed on peripheral B cells, in both humans and mice, and antagonist mAbs to BAFF-R blocked BAFF-mediated costimulation of anti- micro responses. The other BAFF receptors showed a much more restricted expression pattern, suggestive of specialized roles.

Recombinant Human B Lymphocyte Stimulator is a single, non-glycosylated polypeptide chain containing 153 amino acids and having a molecular mass of 17,007 Daltons.

E. coli

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Val-Gln-Gly.

Biological Activity:
Recombinant B Lymphocyte Stimulator is fully biologically active when compared to standard. The activity is determined by the dose-dependent stimulation of IL-8 production by human PBMC. The ED50 for this effect is less than 10ng/ml. Please note: results may vary with PBMC donors.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280.
2. Analysis by RP-HPLC, using a standard solution of BAFF as a Reference Standard.

Reconstitute the lyophilized BAFF in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder in mannitol, HSA.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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4. Laird MW, Sampey GC, Zukauskas D, Li Y, Protein Expr Purif 2005 Feb;39(2):237-46.
5. Gottenberg JE, Busson M, Lavie F, Mariette X, Ann Rheum Dis 2005 Jan 7.
6. Castigli E, Wilson SA, Dedeoglu F, Jabara H, J Exp Med 2005 Jan 3;201(1):35-9.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.