Technical Data
BAFF, Recombinant, Human (B cell Activating Factor belonging to TNF Family, BLyS, TALL-1, THANK)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant corresponding to 153aa from human BAFF expressed in E. coli.

Molecular Weight:

Endotoxin: ~0.1ng/ug

Biological Activity:
ED50=5-10ng/ml, determined by the dose dependent stimulation of IL-8 production by human PBMCs. Please note that this biological activity may vary with each PBMC donor. The biological activity was also determined in a mouse splenocyte survival assay. The ED50 for this assay is 0.5 - 2.0 g/mL. BAFF is a member of the TNF ligand superfamily, which serves as a costimulator of B cell proliferation and function. This recombinant protein corresponds to the TNF-like portion of BAFFs extracellular domain.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli.
Purity: ~95% (SDS PAGE, HPLC)
Concentration: Not determined
Form: Lyophilized from 5 mM Tris, pH 8.0, plus 3 mM NaCl. Reconstitute with sterile dH2O to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.