Technical Data
B3GNT1 (N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase, I-beta-1,3-N-acetylglucosaminyltransferase, iGnT, Poly-N-acetyllactosamine Extension Enzyme, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1, B3GNT6)
B3GNT1 is a 50kD member of the b3GnT family of enzymes. It is synthesized by sensory neurons, endothelial cells and skeletal muscle. In the trans-Golgi, it collaborates with B4GALT1 to generate a linear poly-N-acetyllactosamine sequence that can be incorporated into either N-linked or O-linked glycans. Human B3GNT1 is a 451aa type II transmembrane protein. It contains an 8aa N-terminal cytoplasmic segment, an extended 28aa transmembrane region aa9-36, and a 379aa luminal domain aa37-415. Over aa37-415, human B3GNT1 shares 97% aa identity with mouse B3GNT1.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2B12K14Affinity Purified
100ug-20CBlue IceHumanMouse
~0.5mg/ml after reconstitution
Recombinant corresponding to aa43-415 from human B3GNT1 expressed in CHO cell line.
Purified by Protein A/G affinity chromatography.
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Recognizes human B3GNT1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.