Technical Data
B0072-05
Band 3, phosphorylated (Tyr359) (Band 3 Anion Exchange Protein, Band-3 Anion Transport Protein, BND3, Anion Exchange Protein 1, Anion Exchanger 1, AE1, Erythrocyte Membrane Protein Band 3 Diego Blood Group, CD233, CD233 Antigen, DI, Erythrocyte Membrane P
Description:
The AE1 (anion exchanger, band 3) protein is expressed in erythrocytes and in the A-type intercalated cells of the kidney distal collecting tubule. In both cell types it mediates the electroneutral transport of chloride and bicarbonate ions across the lipid bilayer, and, in erythrocytes (1). AE1 is phosphorylated on Tyr359 and Tyr404 by Lyn in erythrocytes. Phosphorylation of these residues is necessary for correct localization and recycling of kidney AE1 to the basolateral membrane (2).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:50,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9H297Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A phospho-specific peptide corresponding to residues surrounding tyrosine 359 of human AE1.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% glycerol, 0.01% sodium azide and 0.05% BSA.
Specificity:
Recognizes human AE1 when phosphorylated at Tyr359.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sahr KE, et al. Genomics 24(3):491-501, 1994 2. Toye AM et al. Biochem Soc Symp 72:47—63, 200