Technical Data
B0076-12
BAP1 (BRCA1 Associated Protein 1, Ubiquitin carboxyl-terminal hydrolase BAP1, EC 3.4.19.12, BRCA1-associated protein 1, Cerebral protein 6, BAP-1)
Description:
Mutations within the BRCA1 gene, localized to chromosome 17q, are believed to account for approximately 45% of families with increased incidence of both early-onset breast cancer and ovarian cancer. The BRCA1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1863 amino acids. This protein contains a RING domain near the N-terminus and appears to encode a tumor suppressor. BARD1 (BRCA1-associated RING domain protein 1) and BAP1 (BRCA1-associated protein 1) have both been shown to bind to the N-terminus of BRCA1 and are potential mediators of tumor suppression. BARD1 contains an N-terminal RING domain and three tandem ankyrin repeats. The C-terminus of BARD1 contains a region with sequence homology to BRCA1, termed the BRCT domain. BAP1 is a ubiquitin hydrolase and has been shown to enhance BRCA1-mediated cell growth suppression.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa whole cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k8B66Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified full-length human BAP1 expressed in E. coli. Epitope: Within the UCH domain of BAP1. Cellular Localization: Nuclear.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in Tris-glycine, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human BAP1 at ~85kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Harbour JW, Onken MD, Roberson ED, et al. (2010). 330(6009):1410-3.