		Technical Data
B0085-01	BARD1 (BRCA1 Associated Ring Domain 1)

Description:
BARD1 possesses an N-terminal RING motif and the C-terminal BRCT domain. As the name suggests, this protein is the major cellular binding protein of BRCA1. BARD1 is thought to play a key role in regulating BRCA1 stability, localisation and function. BARD1 is also reported to function as a tumor suppressor, since mutations in this protein have been implicated in a number of breast and ovarian cancers.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:15000
Immunoprecipitation: 10-40ug/mg protein
Optimal dilution determined by the researcher.

Recommended Secondary Reagents:
I1903-30U IgG, H&L, X-adsorbed (HRP) Sheep anti rabbit

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		4°C (-20°C Glycerol)	Blue Ice	Human	Rabbit

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to a region of human BARD1 encoded within exon 4.

Purity:

Purified by affinity chromatography.

Form

Supplied as a liquid in Tris-citrate/phosphate, pH 7.4, 0.09% sodium azide before the addition of glycerol to 40%.

Specificity:

Recognizes human BARD1 (BRCA1 associated Ring Domain 1).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.