Technical Data
B0085-01
BARD1 (BRCA1 Associated Ring Domain 1)
Description:
BARD1 possesses an N-terminal RING motif and the C-terminal BRCT domain. As the name suggests, this protein is the major cellular binding protein of BRCA1. BARD1 is thought to play a key role in regulating BRCA1 stability, localisation and function. BARD1 is also reported to function as a tumor suppressor, since mutations in this protein have been implicated in a number of breast and ovarian cancers.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:15000
Immunoprecipitation: 10-40ug/mg protein
Optimal dilution determined by the researcher.

Recommended Secondary Reagents:
I1903-30U IgG, H&L, X-adsorbed (HRP) Sheep anti rabbit

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to a region of human BARD1 encoded within exon 4.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in Tris-citrate/phosphate, pH 7.4, 0.09% sodium azide before the addition of glycerol to 40%.
Specificity:
Recognizes human BARD1 (BRCA1 associated Ring Domain 1).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.