		Technical Data
B0085-04B	BRCAA1 (AT Rich Interactive Domain 4B (RBP1-like) isoform 1, RBP1-like Protein, Breast Carcinoma-associated Antigen BCAA, Breast Cancer-associated Antigen BRCAA1, Retinoblastoma-binding Protein 1-like 1, SIN3A-associated Protein 180)

Description:
The novel BRCAA1 protein’s epitope, SSKKQKRSHK, was mapped to the cytoplasm of MCF7 cells. Immunohistochemistry analysis has shown that the antigen epitope exhibits positive expression in 65% of breast cancer specimens. Its expression is associated with characteristics that are associated with prognosis of breast cancer, and its sera antibody level may be helpful for breast cancer diagnosis.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1,000
Optimal dilutions to be determined by the researcher.

Positive Control:
MCF-7 human breast cancer cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide, SSKKQKRSHK, corresponding to aa1046-1055 of human BRCAA1 (KLH).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4. No preservative added.

Specificity:

Recognizes human BRCAA1 at ~136kD. Species Crossreactivity: mouse, rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Cui, D., et al., (2004), Cancer Epidemiol Biomarkers Pres., 13: 1136-1145.