Technical Data
B0085-04B
BRCAA1 (AT Rich Interactive Domain 4B (RBP1-like) isoform 1, RBP1-like Protein, Breast Carcinoma-associated Antigen BCAA, Breast Cancer-associated Antigen BRCAA1, Retinoblastoma-binding Protein 1-like 1, SIN3A-associated Protein 180)
Description:
The novel BRCAA1 protein’s epitope, SSKKQKRSHK, was mapped to the cytoplasm of MCF7 cells. Immunohistochemistry analysis has shown that the antigen epitope exhibits positive expression in 65% of breast cancer specimens. Its expression is associated with characteristics that are associated with prognosis of breast cancer, and its sera antibody level may be helpful for breast cancer diagnosis.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1,000
Optimal dilutions to be determined by the researcher.

Positive Control:
MCF-7 human breast cancer cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide, SSKKQKRSHK, corresponding to aa1046-1055 of human BRCAA1 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. No preservative added.
Specificity:
Recognizes human BRCAA1 at ~136kD. Species Crossreactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cui, D., et al., (2004), Cancer Epidemiol Biomarkers Pres., 13: 1136-1145.