Technical Data
Barmotin (Tight Junction-associated Protein)
Tight junctions function to maintain cellular polarity and permeability barriers in epithelial and endothelial cells. Several molecules of the tight junction complex have been identified. Studies with Madin-Darby canine kidney (MDCK) cells indicate the 7H6 antigen to be an important molecule for regulation of the barrier function of tight junctions. Confocal laser scanning microscopy revealed that the expression of 7H6 antigen decreased sequentially during hepatocarcinogenesis. The 7H6 antigen was expressed intensely at the apical and basolateral membrane of cancer cells with glandular pattern. Examination of human gastric and colon cancer tissues showed that tight junctions were maintained highly in the well-differentiated (gland-forming) adenocarcinomas, but reduced in poorly-differentiated adenocarcinomas. In MDCK cells the disappearance of 7H6 expression is closely related with cell spreading in vitro induced hepatocyte growth factor/scatter factor.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:10
Immunohistochemistry (Frozen): 1:10
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgM7H6Affinity Purified
100ug4CBlue IceHumanMouse
Human Barmotin
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.1% BSA.
Recognizes human Barmotin at ~155kD. Localized at the periphery of tight junctions of hepatocytes and other epithelia and in the intestine and kidney. Species Crossreactivity: Canine, mouse, rat and chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zhong, Y et al; Monoclonal antibody 7H6 reacts with a novel tight junction-associated protein distinct from ZO-cingulin and ZO-2. J Cell Biol 1993, 120: 477. 2. Kojima, T et al; Sequential changes in intercellular junctions between hepatocytes during the course of acute liver injury and restoration after thioacetamide treatment. Virchos Arch 1994, 425: 407. 3. Zhong, Y et al; Sequential decrease in tight junctions as revealed by 7H6 tight junction-associated protein during rat hepatocarcinogenesis. Jpn J Cancer Res 1994, 85: 351. 4. Zhong, Y et al; Localization of the 7H6 antigen at tight junctions correlates with the paracellular barrier function of MDCK cells. Exp Cell Res 1994, 214: 614. 5. Kimura, M et al; Comparison between the distribution of 7H6 tight junction-associated antigen and occludin during the development of chick intestine. Cell Struct Funct 1996, 21: 91. 6. Kimura, H et al; Bacterial lipopolysaccharide reduced intestinal barrier function and altered localization of 7H6 antigen in IEC-6 rat intestinal crypt cells. J Cell Physiol 1997, 171: 284. 7. Muto, S et al; HGF/SF-induce spreading of MDCK cells correlates with disappearance of barmotin/7H6, a tight junction-associated protein, from the cell membrane. Cell biol Int 2000, 24:439.