Technical Data
Media
Basal Medium Eagle (BME) w/Earle’s, L-Glutamine, HEPES (Powder)
B0100
Cell Culture Grade
Storage RT    Shipping RT
Components shown as g/liter
Inorganic Salts:
Calcium Chloride•2H2O0.265
Magnesium Sulfate0.09767
Potassium Chloride0.4
Sodium Chloride6.8
Sodium Phosphate Mono0.122
Amino Acids:
L-Arginine•HCl0.021
L-Cystine•2HCl0.01565
L-Glutamine0.292
L-Histidine0.008
L-Isoleucine0.026
L-Leucine0.026
L-Lysine•HCl0.03647
L-Methionine0.0075
L-Phenylalanine0.0165
L-Threonine0.024
L-Tryptophan0.004
L-Tyrosine•2Na•2H2O0.02595
L-Valine0.0235
Vitamins:
D-Biotin0.001
Choline Chloride0.001
Folic Acid0.001
myo-Inositol0.002
Niacinamide0.001
D-Pantothenic Acid•Ca0.001
Pyridoxal•HCl0.001
Riboflavin0.0001
Thiamine•HCl0.001
Other:
D-Glucose1
HEPES5.958
Phenol Red, Sodium0.011
Total:14.4g/L
Basal Medium Eagle (BME) developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. BME was developed a result of numerous studies carried out in the late 1950’s to determine the essential nutritional requirements and other factors critical to the growth of cells in culture. BME, when properly supplements, has demonstrated wide applicability, for supporting monolayer growth of a wide variety of normal and transformed cell lines. BME is the predecessor of Eagle’s Minimum Essential Medium (MEM) and Dulbecco’s Modified Eagle’s Medium (DME). BME is suitable for use with diploid and primary mammalian cell cultures.


Directions per Liter: Dissolve 14.4g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2.2g sodium bicarbonate. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: Homogenous, free flowing powder.

Solubility (14.4g/L):

pH:

Endotoxin: ~10EU/mg


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H., et al., J. Biol. Chem. 214: 845-847 (1956). 2. Eagle, H., Tissue Culture Association Manual 3: 517-520 (1976). 3. Eagle, H., Science 130: 432-437 (1959). 4. Eagle, H., Science 122: 501 (1955).