Technical Data
Media
Basal Medium Eagle (BME) 10X w/Earle’s w/o L-Glutamine (Powder)
B0101-06
Cell Culture Grade
Storage RT    Shipping RT
Components shown as mg/Liter
1X10X
Inorganic Salts:
Calcium Chloride•2H2O264.92649
Magnesium Sulfate 97.7977
Potassium Chloride4004000
Sodium Chloride680068000
Sodium Phosphate Mono•H2O1401400
Amino Acids:
L-Arginine•HCl21.06210.6
L-Cystine12.01120.1
L-Histidine•HCl•H2O10.5105
L-Isoleucine26.23262.3
L-Leucine26.23262.3
L-Lysine•HCl36.53365.3
L-Methionine7.4674.6
L-Phenylalanine16.51165.1
L-Threonine23.82238.2
L-Tryptophan4.0840.8
L-Tyrosine18.1181
L-Valine23.43234.3
Vitamins:
D-Biotin110
Choline Chloride110
Folic Acid110
i-Inositol220
Nicotinamide110
D-Pantothenic Acid•Ca110
Pyridoxal•HCl110
Riboflavin0.11
Thiamine•HCl110
Other:
D-Glucose100010000
Phenol Red, Sodium17170
Total:8.9689.6 g/Liter
Basal Medium Eagle (BME) developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. BME was developed a result of numerous studies carried out in the late 1950’s to determine the essential nutritional requirements and other factors critical to the growth of cells in culture. BME, when properly supplements, has demonstrated wide applicability, for supporting monolayer growth of a wide variety of normal and transformed cell lines. BME is the predecessor of Eagle’s Minimum Essential Medium (MEM) and Dulbecco’s Modified Eagle’s Medium (DME). BME is suitable for use with diploid and primary mammalian cell cultures.


Directions per Liter of 10X: Dissolve 89.6 grams per liter of distilled/deionized (DDI) water. Heat with stirring until completely solubilized. Adjust pH as necessary. Sterilize with 0.2um membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Note: Some precipitate may be observed due to excess concentration of salts. Sterile filter after solubilization. USB recommends the preparation of this product as a 1X solution.Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Homogenous, free flowing powder.

Solubility: Some precipitate may be observed due to excess concentration of salts. Sterile filter after solubilization.

10EU/mg:


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H., et al., J. Biol. Chem. 214: 845-847 (1956). 2. Eagle, H., Tissue Culture Association Manual 3: 517-520 (1976). 3. Eagle, H., Science 130: 432-437 (1959). 4. Eagle, H., Science 122: 501 (1955).