Technical Data
Media
Basal Medium Eagle (BME) w/ Hanks’, L-Glutamine, HEPES (Powder)
B0102
Cell Culture Grade
Storage RT    Shipping RT
Components shown as g/Liter
Inorganic Salts:
Calcium Chloride0.18545
Potassium Chloride0.400
Magnesium Sulfate0.060
Sodium Chloride8.000
Sodium Phosphate Dibasic0.04769
Amino Acids:
L-Arginine0.02116
L-Cystine•2HCl0.01565
L-Glutamine0.292
L-Histidine•HCl0.01046
L-Isoleucine0.026
L-Leucine0.026
L-Lysine•HCI0.03623
L-Methionine0.0075
L-Phenylalanine0.0165
L-Threonine0.024
L-Tryptophan0.004
L-Tyrosine•2Na0.026
L-Valine0.0234
Vitamins:
Biotin0.001
D-Pantothenic Acid•Ca0.001
Choline Chloride0.001
Folic Acid0.001
i-Inositol0.002
Nicotinamide0.001
Pyridoxal HCI0.001
Riboflavin0.0001
Thiamine HCI0.001
Other:
D-Glucose1.000
HEPES Free Acid5.958
Pheno lRed0.0102
Total:16.2g/liter
Basal Medium Eagle (BME) developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. BME was developed a result of numerous studies carried out in the late 1950’s to determine the essential nutritional requirements and other factors critical to the growth of cells in culture. BME, when properly supplements, has demonstrated wide applicability, for supporting monolayer growth of a wide variety of normal and transformed cell lines. BME is the predecessor of Eagle’s Minimum Essential Medium (MEM) and Dulbecco’s Modified Eagle’s Medium (DME). BME is suitable for use with diploid and primary mammalian cell cultures.


Directions per Liter: Dissolve 16.2g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2.2g sodium bicarbonate. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: Homogenous, free flowing powder.

Solubility (16.2g/L):

pH: As reported

Endotoxin: ~10EU/mg


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H., et al., J. Biol. Chem. 214: 845-847 (1956). 2. Eagle, H., Tissue Culture Association Manual 3: 517-520 (1976). 3. Eagle, H., Science 130: 432-437 (1959). 4. Eagle, H., Science 122: 501 (1955).