Technical Data
Media
Basal Medium Eagle (BME) w/ Hanks’, HEPES w/o L-Glutamine (Powder)
B0105
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride•2H2O264.90
Magnesium Sulfate97.70
Potassium Chloride400.00
Sodium Chloride6800.00
Sodium Phosphate Monobasic140.00
Amino Acids:
L-Arginine21.06
L-Cystine12.01
L-Histidine•HCl•H2O10.50
L-Isoleucine26.23
L-Leucine26.23
L-Lysine•HCI36.53
L-Methionine7.46
L-Phenylalanine16.51
L-Threonine23.82
L-Tryptophan4.08
L-Tyrosine18.11
L-Valine23.43
Vitamins:
Biotin1.00
Choline Chloride1.00
Folic Acid1.00
i-Inositol2.00
Nicotinamide1.00
D-Pantothenic Acid•Ca1.00
Pyridoxal HCI1.00
Riboflavin0.10
Thiamine HCI1.00
Other:
D-Glucose1000.00
HEPES Free Acid4770.00
Phenol Red•Na17.00
Total (g/L):13.7
Basal Medium Eagle (BME) developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. BME was developed a result of numerous studies carried out in the late 1950’s to determine the essential nutritional requirements and other factors critical to the growth of cells in culture.

BME, when properly supplemented, has demonstrated wide applicability, for supporting monolayer growth of a wide variety of normal and transformed cell lines. BME is the predecessor of Eagle’s Minimum Essential Medium (MEM) and Dulbecco’s Modified Eagle’s Medium (DME). BME is suitable for use with diploid and primary mammalian cell cultures.


Directions per Liter: Dissolve 13.7g in 800-900ml of ddH2O, stirring gently until completely solubilized. Add 2.2g sodium bicarbonate. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light pink to orange, homogenous, free flowing powder

Solubility (13.7g/L): Yellow, clear, complete

pH: As reported

Endotoxin: ~10EU/mg


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H., et al., J. Biol. Chem. 214: 845-847 (1956). 2. Eagle, H., Tissue Culture Association Manual 3: 517-520 (1976). 3. Eagle, H., Science 130: 432-437 (1959). 4. Eagle, H., Science 122: 501 (1955).