Technical Data
Media
Basal Medium Eagle (BME) 100X Amino Acids, w/o Glutamine (Powder)
B0109
Cell Culture Grade
Storage RT    Shipping RT
Components shown as mg/L
Amino Acids:
L-Arginine•HCl2106
L-Cystine•Na2•H201511
L-Histidine•HCl•H2O1050
L-Isoleucine2623
L-Leucine2623
L-Lysine•HCl3653
L-Methionine746
L-Phenylalanine1651
L-Threonine2382
L-Tryptophan408
L-Tyrosine•Na2•H2O2595
L-Valine2343
Total:23.7g/L
Basal Medium Eagle (BME) developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. BME was developed a result of numerous studies carried out in the late 1950’s to determine the essential nutritional requirements and other factors critical to the growth of cells in culture. BME, when properly supplements, has demonstrated wide applicability, for supporting monolayer growth of a wide variety of normal and transformed cell lines. BME is the predecessor of Eagle’s Minimum Essential Medium (MEM) and Dulbecco’s Modified Eagle’s Medium (DME). BME is suitable for use with diploid and primary mammalian cell cultures.


Directions per Liter: Dissolve 23.7 grams per liter of distilled/deionized (DDI) water, stirring until completely solubilized. Filter-sterilize with 0.2um membrane filter. Aliquot into sterile containers.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: White, homogenous, free flowing powder.

Solubility (1X): Colorless, clear, complete

pH: As reported

Endotoxin: ~10EU/mg


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H. et al., J. Biol. Chem. 214: 845-847 (1956) 2. Eagle, H., Tissue Culture Association Manual 3: 517-520 (1976) 3. Eagle, H., Science 130: 432-437 (1959).4. Eagle, H., Science 122: 501 (1955)