Technical Data
Media
Basal Medium Eagle (BME) 100X Vitamins (Powder)
B0110
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/L
1X10X100X
Vitamins:
D-Biotin110100
Choline Chloride110100
Folic Acid110100
i-Inositol220200
Nicotinamide110100
D-Pantothenic Acid•Ca110100
Pyridoxal•HCl110100
Riboflavin0.1110
Thiamine•HCl110100
Total (g/L):0.00910.0910.91
Eagle’s Basal Medium (BME) was developed by Harry Eagle. BME forrmula was developed as a result of numerous studies carried out in the late 1950’s to determine the essential nutritional requirements and other factors critical to the growth of cells in culture. Historically, BME has been used in studies conducted to measure the growth response of normall (WI-38) and transformed (mouse and HeLa) cells in monolayer growth. It is one of the most widely used of all synthetic cell culture media.


Directions per Liter: To prepare a 100X stock solution, dissolve 0.91g in 800-900ml of ddH2O or BME media, stirring gently with heat until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.Dilute 1:100 with BME media prior to use.

Alternatively:1. To prepare 10X stock solution, add 0.09g to dH2O or BME media. Dilute 1:10 with BME media.2. To prepare 1X working solution, add 0.009g to dH2O or BME media.Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Tan to yellow, homogenous, free flowing powder. Solubility (1X)


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H., et al., J. Biol. Chem. 214: 845-847 (1956). 2. Eagle, H., Tissue Culture Association Manual 3: 517-520 (1976). 3. Eagle, H., Science 130: 432-437 (1959). 4. Eagle, H., Science 122: 501 (1955).