Technical Data
Basic Hair Keratin, K82 (KRT82, Keratin 82, HB2, Hb-2, K82, Keratin, type II cuticular Hb2, Keratin-82, KRTHB2, Type II hair keratin Hb2)
Type II hair keratin is one of the two types of hair keratin. It is a basic protein which heterodimerizes with type I hair keratins to form hair and nails.

Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): 1:200 for 1 hour at RT
Western Blot (ECL): 1:2000 for 1 hour at RT.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanGuinea pig
Not Determined
Synthetic peptide corresponding to human basic hair (trichocytic) keratin K82, conjugated to KLH. (Formally designated keratin hHb2; SIV GTG ELY VPC EPQ GLL SC)
Supplied as a liquid, 0.09% sodium azide.
Recognizes human hair keratin K82 present starting from the mid bulb region in the hair cuticle (contrary to K32 expression, starting from the lower part of the cuticle). Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Langbein L et al., J Biol. Chem 276: 35123-35132 (2001). 2. Langbein L., et al., Methods in Cell Biology 78: 413-456 (2004).