Technical Data
B0130-20
Basic Hair Keratin, K82 (KRT82, Keratin 82, HB2, Hb-2, K82, Keratin, type II cuticular Hb2, Keratin-82, KRTHB2, Type II hair keratin Hb2)
Description:
Type II hair keratin is one of the two types of hair keratin. It is a basic protein which heterodimerizes with type I hair keratins to form hair and nails.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): 1:200 for 1 hour at RT
Western Blot (ECL): 1:2000 for 1 hour at RT.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanGuinea pig
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to human basic hair (trichocytic) keratin K82, conjugated to KLH. (Formally designated keratin hHb2; SIV GTG ELY VPC EPQ GLL SC)
Purity:
Serum
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes human hair keratin K82 present starting from the mid bulb region in the hair cuticle (contrary to K32 expression, starting from the lower part of the cuticle). Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Langbein L et al., J Biol. Chem 276: 35123-35132 (2001). 2. Langbein L., et al., Methods in Cell Biology 78: 413-456 (2004).