Technical Data
B0130-23
Basic Hair Keratin, K83 (KRT83, Keratin 83, HB3, Hb-3, K2.10, K83, Keratin, type II cuticular Hb3, Keratin-83, KRTHB3, MGC141663, Type II hair keratin Hb3)
Description:
Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen): 1:200; Specifically stains human hair keratin KB3 expressed starting from the middle cortex. For enhancement of cortex staining perincubate fixed sections with PBS, 0.1 Triton X-100 for 1-5 minutes prior to first incubation. Incubate 1 hour at RT.
Western Blot (ECL): 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanGuinea pig
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to human basic hair keratin K83 (hHb3), CGFNSIGCGFRPGNF (KLH).
Purity:
Serum
Form
Supplied as a liquid with 0.09% sodium azide.
Specificity:
Recognizes human Basic Hair Keratin, K83. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Langbein L, Rogers M, Winter H, Prätzel S, Schweizer J. J Biol Chem 276, 35123-35132 (2001). 2. Langbein L, Spring H, Rogers MA, Praetzel S, Schweizer J. Methods in Cell Biology 78, 413-456 (2004).