		Technical Data
B0130-23	Basic Hair Keratin, K83 (KRT83, Keratin 83, HB3, Hb-3, K2.10, K83, Keratin, type II cuticular Hb3, Keratin-83, KRTHB3, MGC141663, Type II hair keratin Hb3)

Description:
Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen): 1:200; Specifically stains human hair keratin KB3 expressed starting from the middle cortex. For enhancement of cortex staining perincubate fixed sections with PBS, 0.1 Triton X-100 for 1-5 minutes prior to first incubation. Incubate 1 hour at RT.
Western Blot (ECL): 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Guinea pig

Concentration:

Not Determined

Immunogen:

Synthetic peptide corresponding to human basic hair keratin K83 (hHb3), CGFNSIGCGFRPGNF (KLH).

Purity:

Serum

Form

Supplied as a liquid with 0.09% sodium azide.

Specificity:

Recognizes human Basic Hair Keratin, K83. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Langbein L, Rogers M, Winter H, Prätzel S, Schweizer J. J Biol Chem 276, 35123-35132 (2001). 2. Langbein L, Spring H, Rogers MA, Praetzel S, Schweizer J. Methods in Cell Biology 78, 413-456 (2004).