Technical Data
B0130-26
Basic Hair Keratin, K84 (KRT84, Keratin 84, HB4, Hb-4, K84, Keratin, Type II Cuticular Hb4, Keratin-84, KRTHB4, Type II Hair Keratin Hb4)
Description:
Type II hair keratin is one of the two types of hair keratin. It is a basic protein which heterodimerizes with type I hair keratins to form hair and nails.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): 1:200. Incubate at RT for 1 hour.
Western Blot (ECL): 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanGuinea pig
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to human basic hair (trichocytic) keratin K84, conjugated to KLH. (Formerly designated hHb4; IAA VGS RPI HCG VRF).
Purity:
Serum
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes human hair keratin K84 expressed starting from the upper layers of the filiform papillae of tongue epithelium and in mouse tail buds. No staining of anagen hair follicle. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Langbein, L., et al., J. Biol. Chem. 276: 35,123-35,132 (2001). 2. Langbein, L., et al., Methods in Cell Biology 78: 413-456 (2004).