		Technical Data
B0130-29	Basic Hair Keratin, K85 (KRT85, Keratin 85, HB5, Hb-5, hHb5, K12.12, K85, Keratin, type II cuticular Hb5, KRTHB5, Type II hair keratin Hb5)

Description:
Type II hair keratin is one of the two types of hair keratin. It is a basic protein which heterodimerizes with type I hair keratins to form hair and nails.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen): 1:200. Preincubate fixed sections with 0.1 Triton X-100 (in PBS) for 1-5 minutes prior to first incubation step. Incubation time: 1 hour at RT.
Western Blot (ECL): 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Guinea pig

Concentration:

Not Determined

Immunogen:

Synthetic peptide corresponding to GRQ IST GPS AIG GSI TVV APD C of human basic hair (trichocytic) keratin K85 (hHb5) (KLH).

Purity:

Serum

Form

Supplied as a liquid, 0.09% sodium azide.

Specificity:

Recognizes human hair keratin hHb5 expressed starting from the hair upper matrix, cortex and hair cuticle. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Langbein L. et al., J Biol chem 276: 35123-35132 (2001). 2. Langbein L. et al, Method in cell Biology 78: 413-456 (2004)