Technical Data
B0130-29
Basic Hair Keratin, K85 (KRT85, Keratin 85, HB5, Hb-5, hHb5, K12.12, K85, Keratin, type II cuticular Hb5, KRTHB5, Type II hair keratin Hb5)
Description:
Type II hair keratin is one of the two types of hair keratin. It is a basic protein which heterodimerizes with type I hair keratins to form hair and nails.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen): 1:200. Preincubate fixed sections with 0.1 Triton X-100 (in PBS) for 1-5 minutes prior to first incubation step. Incubation time: 1 hour at RT.
Western Blot (ECL): 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanGuinea pig
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to GRQ IST GPS AIG GSI TVV APD C of human basic hair (trichocytic) keratin K85 (hHb5) (KLH).
Purity:
Serum
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes human hair keratin hHb5 expressed starting from the hair upper matrix, cortex and hair cuticle. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Langbein L. et al., J Biol chem 276: 35123-35132 (2001). 2. Langbein L. et al, Method in cell Biology 78: 413-456 (2004)