Technical Data
B0130-32
Basic Hair Keratin, K86 (KRT86, Keratin 86, FLJ25176, Hb1, Hb6, HB6, hHb6, K2.11, K86, Keratin, type II cuticular Hb6, Keratin-86, KRTHB1, KRTHB6, MNX, Type II Hair Keratin Hb6)
Description:
Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen): 1:200. Preincubate fixed sections with 0.1 Triton X-10 (in PBS) for 1-5 min prior to 1 hour incubation.
Western Blot: 1:2000. Incubate for 1 hour at RT.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanGuinea pig
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to human basic hair keratin K86, APV VST RVS SVP SNS NVV VGT TNA C (KLH).
Purity:
Serum
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes human hair keratin (hHb6) expressed starting from the middle hair cortex. It can be defect in monilethrix. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.