		Technical Data
B0130-32	Basic Hair Keratin, K86 (KRT86, Keratin 86, FLJ25176, Hb1, Hb6, HB6, hHb6, K2.11, K86, Keratin, type II cuticular Hb6, Keratin-86, KRTHB1, KRTHB6, MNX, Type II Hair Keratin Hb6)

Description:
Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen): 1:200. Preincubate fixed sections with 0.1 Triton X-10 (in PBS) for 1-5 min prior to 1 hour incubation.
Western Blot: 1:2000. Incubate for 1 hour at RT.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Guinea pig

Concentration:

Not determined

Immunogen:

Synthetic peptide corresponding to human basic hair keratin K86, APV VST RVS SVP SNS NVV VGT TNA C (KLH).

Purity:

Serum

Form

Supplied as a liquid, 0.09% sodium azide.

Specificity:

Recognizes human hair keratin (hHb6) expressed starting from the middle hair cortex. It can be defect in monilethrix. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.