Technical Data
B0140
Bassoon
Description:
Bassoon is a 420kD protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle (1-3). Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96% (1). The human BASSOON gene has recently been cloned and localized (3). Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains (1). The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration (1,3). Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors (2). Another presynaptic protein, Piccolo (4) was found to colocalize with Bassoon in cultured hippocampal neurons (1). These observations suggested that they serve specific functions at synaptic junctions and
may be involved in organization of the cytoskeleton at the site of neurotransmitter release (1).

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot (ECL): 1ug/ml
Immunohistochemistry: 2-5ug/ml
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Rat brain tissue extract.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a4E15Affinity Purified
SizeStorageShippingSourceHost
25ug4°C (-20°C Glycerol)Blue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant rat bassoon fragment.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity:
Recognizes an ~400kD protein, corresponding to the apparent molecular mass of rat Bassoon. Additional bands between 97 and 400kD are also identified and are reported to be proteolytic degradation products of Bassoon. By immunoctyochemical analysis, this antibody displays a punctate distribution on cultured hippocampal neurons similar to that seen for synaptophysin. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tom Dieck, S., Sanmarti-Vila, L., Langnaese, K., Richter, K, Kindler, S., Soyke, A., Wex, H, Smalla, K-H., Kampf, U., Franzer, J-T., Stumm, M., Garner, C.C., Gundelfinger, E.D. (1998) J. Cell Biol. 142: 499-509. 2. Richter, K., Langnaese, K., Kreutz, M.R., Olias, G., Zhai, R., Scheich, H., Garner, C.C., Gundelfinger, E.D. (1999) J. Comp. Neurol. 408: 437-448. 3. Winter, C., tom Dieck, S., Boeckers, T.M., Bockmann, J., Kanmpf, U., Sanmarti-Vila, L., Langnaese, K., Altrock, W., Stumm, M., Soyke, A., Wieacker, P., Garner, C.C., Gundelfinger, E.D. (1999) Genomics 57: 389-397. 4. Cases-Langhoff, C., Voss, B, Garner, A.M., Appeltauer, U., Takei, K., Kindler, S., Veh,
R.W., De Camilli, P., Gundelfinger, E.D., Garner, C.C. (1996) Eur. J. Cell Biol 69: 214-223. 5. Phillips, G.R., et al. (2001) Neuron 32: 63-77. 6. Morales, M., Colicos, M.A., Goda, Y. (2000) Neuron 27: 539-550. 7. Ohtsuka, T.; Takao-Rikitsu, E.; Inoue, E.; Inoue, M.; Takeuchi, M.; Matsubara, K.; Deguchi-Tawarada, M., Satoh, K., Morimoto, K., Nakanishi, H., Takai, Y. (2002) J Cell Biol 158(3): 577-590. 8. Shiraishi, Y., Mizutani, A., Mikoshiba, K., Furuichi, T. (2003) Mol Cell Neurosci 22(2):
188-201.