Technical Data
B0290-05
BCKDHA, CT (2-oxoisovalerate Dehydrogenase Subunit alpha, Mitochondrial, Branched-chain alpha-keto Acid Dehydrogenase E1 Component alpha Chain, BCKDH E1-alpha, BCKDE1A)
Description:
The branched-chain alpha-keto acid (BCAA) dehydrogenase(BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). BCKDHA is the alpha subunit of the decarboxylase (E1) component.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the C-terminal region of human BCKDHA (KLH).
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS, 0.123% sodium azide.
Specificity:
Recognizes human BCKDHA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Flaschker,N., et.al., J. Inherit. Metab. Dis. 30(6), 903-909 (2007).