Technical Data
B0395
BCA-1 Human, Recombinant (B Cell Attracting Chemokine1, B Lymphocyte Chemoattractant, BLC, CXCL13, BLR1 Ligand)
5ug
20ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
BCA-1/BLC, a CXC chemokine, is expressed in the liver, spleen, lymph nodes, appendix and stomach. It exerts its activities through its only receptor CXCR5. BCA-1/BLC is a potent chemoattractant for B lymphocytes and induces weak chemotactic response in T cells and macrophages. It manifests no activity on neutrophils and monocytes. Recombinant Human BCA-1 is a 10.3kD protein containing 85 amino acid residues including the four highly conserved cysteine residues present in CXC chemokines.

AA Sequence:
VLEVYYTSLR CRCVQESSVF IPRRFIDRIQ ILPRGNGCPR KEIIVWKKNK SIVCVDPQAE WIQRMMEVLR KRSSSTLPVP VFKRKIP

Biological Activity:
Determined by its ability to chemoattract human B cells using a concentration range of 1-10ng/ml.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with Acetic acid. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
10.3kD
Source: E. coli
Purity: 98% by SDS-PAGE and HPLC. Endotoxin:
0.1ng/ug (1EU/ug)
Form: Supplied as a lyophilized powder with no additives.
Reconstitute with Acetic acid to a concentration of 0.1-1.0mg/ml. Do not vortex. Further dilute with PBS, 0.1% BSA or HSA.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.