Technical Data
B0395-05
BCA-1 Human, Recombinant (B Cell Attracting Chemokine1, B Lymphocyte Chemoattractant, BLC, CXCL13, BLR1 Ligand)
Description:
BCA-1/BLC, a CXC chemokine, is expressed in the liver, spleen, lymph nodes, appendix and stomach. It exerts its activities through its only receptor CXCR5. BCA-1/BLC is a potent chemoattractant for B lymphocytes and induces weak chemotactic response in T cells and macrophages. It manifests no activity on neutrophils and monocytes. Recombinant Human BCA-1 is a 10.1 D protein containing 85 amino acid residues including the four highly conserved cysteine residues present in CXC chemokines.

Applications:
Suitable for use inDirect ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
ELISA: >0.5ug/ml. Detects hBCA-1 at 0.2-0.4ng/well of recombinant hBCA-1.
Western Blot: 0.1-0.2ug/ml. Detects hBCA-1 at 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Highly purified, recombinant human BCA-1 (B Cell-Attracting Chemokine-1)
Purity:
Purified by affinity chromatography
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity:
Recognizes human BCA-1 (B Cell-Attracting Chemokine-1)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.