		Technical Data
B0395-07	BCA-1 (B Cell Attracting Chemokine1, B Lymphocyte Chemoattractant, BLC, CXCL13, BLR1 Ligand)

Description:
BLC (B-lymphocyte chemoattractant, murine equivalent of human B cell-attracting chemokine-1, BCA-1) is a 12kD recently characterized ELR-CXC chemokine. BLC showed high levels of expression in spleen, Peyer's patches, and mesenteric lymph nodes. It is reported as the ligand of CXCR5. It attracts primarily B cells in vitro. Cell lines transfected with CXCR5 demonstrate both chemotaxis and calcium flux in response to BLC.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Mouse	Rabbit

Concentration:

~1mg/ml

Immunogen:

E. coli-expressed mouse BLC

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a lyophilized powder in 0.1% sodium azide.

Specificity:

Reacts with mouse BLC.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Gunn, M., et al. (1998) A B-cell-homing chemokine made in lymphoid follicles activates Burkitt's lymphoma receptor-1. Nature 391:799-803. 2. Legler, D., et al. (1998) B cell attracting chemokine 1, a human CXC chemokine expressed in lymphoid tissues, selectively attracts B lymphocytes via BLR1/CXCR5. J Exp Med. 187(4):655-660.