Technical Data
B0395-07
BCA-1 (B Cell Attracting Chemokine1, B Lymphocyte Chemoattractant, BLC, CXCL13, BLR1 Ligand)
Description:
BLC (B-lymphocyte chemoattractant, murine equivalent of human B cell-attracting chemokine-1, BCA-1) is a 12kD recently characterized ELR-CXC chemokine. BLC showed high levels of expression in spleen, Peyer's patches, and mesenteric lymph nodes. It is reported as the ligand of CXCR5. It attracts primarily B cells in vitro. Cell lines transfected with CXCR5 demonstrate both chemotaxis and calcium flux in response to BLC.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
E. coli-expressed mouse BLC
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder in 0.1% sodium azide.
Specificity:
Reacts with mouse BLC.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gunn, M., et al. (1998) A B-cell-homing chemokine made in lymphoid follicles activates Burkitt's lymphoma receptor-1. Nature 391:799-803. 2. Legler, D., et al. (1998) B cell attracting chemokine 1, a human CXC chemokine expressed in lymphoid tissues, selectively attracts B lymphocytes via BLR1/CXCR5. J Exp Med. 187(4):655-660.