Technical Data
B Cell Linker Protein (BLNK, BASH, BLNK-s, Ly57, MGC111051, SLP-65, SLP65)
Domain Information: Occludin/ELL family; Src homology 2 domains

Gene Ontology Terms: transmembrane receptor protein tyrosine kinase adaptor protein activity; hemocyte development; humoral immune response; intracellular signaling cascade; inflammatory response; cytoplasm

Application(s): Suitable for use in ELISA, Western Blot. Other applications not tested.
Western blot: Approx 70kD band seen in Daudi lysate. Recommended for use at 3-5ug/ml. Please note that although the predicted MW of BLNK is approximately 50kD, it runs at 70-75kD on SDS-PAGE.

Staining (4ug/ml) of Daudi lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See B0397-01B-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
PabIgGAffinity Purified
100ug-20°CBlue IceHumanGoat
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Recognizes Human, Mouse, Rat BLNK
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Fu C, Turck CW, Kurosaki T, Chan AC. , BLNK: a central linker protein in B cell activation., Immunity. 1998 Jul;9(1):93-103.