Technical Data
B0397-01M
BLNK (B cell Linker, BASH, Bca, B Cell Adapter Containing a SH2 Domain Protein, B Cell Adapter Containing a Src Homology 2 Domain Protein, Cytoplasmic Adapter Protein, Lymphocyte Antigen 57, Ly57, Ly-57, Lyw-57, Src Homology 2 Domain-containing Leukocyte
Description:
BLNK (B cell linker protein) is an adaptor molecule that plays key role in B cell activation initiated by cross-linking the B cell receptor (BCR). This activation leads to an increase in the tyrosine phosphorylation of a number of proteins due to activation of nonreceptor protein tyrosine kinases (PTK) including Syk, which is responsible for the tyrosine phosphorylation of BLNK. BLNK is cytosolic protein identified as two phosphoproteins migrating at 68 and 70kD in SDS/PAGE (alternatively spliced forms of human BLNK). Mapping of the tyrosine residues in BLNK reveal three tyrosines that bind PLC-gamma2, and two tyrosines that bind the Tec family kinase Btk, the Vav nucleotide exchange factor and the adaptor Nck.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
Fusion protein corresponding to aa71-356 of human BLNK.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity:
Recognizes mouse BLNK.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.