Technical Data
B0397-11
B Cell Linker Protein (BLNK, BASH, BLNK-s, Ly57, MGC111051, SLP-65, SLP65)
Description:
B-cell linker protein (BLNK, also known as SLP65, BASH, and BCA) is a B cell adaptor that plays an important role in B cell development and in BCR-signal transduction. Since BLNK does not encode any intrinsic enzymatic activity, its function is to serve as a scaffold for assembling macromolecular complexes which includes enzymes (PLC gamma, Vav and Btk) and additional linker proteins (Grb2 and Nck) (1-3) . In addition, BLNK binds Btk and is required for activation of the transcription factor NF- B. Tyrosine phosphorylation of BLNK by Syk provides specific docking sites for these SH2-containing effector proteins which in turn permits phosphorylation and/or activation of their respective signaling pathways.

Applications:
Suitable for use in Western Blotting, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blotting: 1:500
Immunohistochemistry: 1:50
Immunoprecipitation: 1:20

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG6k39Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Immunogen:
A synthetic peptide corresponding to residues near the C-term of human BLNK
Purity:
Supernatant
Form
Supplied as a liquid
Specificity:
Recognizes human BLNK
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fu C., Turck,C.,et al. (1998) Immunity, 9, 93–103 2. Jumaa H., et al (2001) Eur. J. Immunol., 31, 2164–2169. 3. Hashimoto S. et al. (1999). Blood, 94, 2357–2364 4. Christopher W. et al EMBO J. 2002 December 2; 21(23): 6461–6472.