Technical Data
B0397-11
B Cell Linker Protein (BLNK, BASH, BLNK-s, Ly57, MGC111051, SLP-65, SLP65)
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Technical Data |
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B0397-11 |
B Cell Linker Protein (BLNK, BASH, BLNK-s, Ly57, MGC111051, SLP-65, SLP65) |
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Description: B-cell linker protein (BLNK, also known as SLP65, BASH, and BCA) is a B cell adaptor that plays an important role in B cell development and in BCR-signal transduction. Since BLNK does not encode any intrinsic enzymatic activity, its function is to serve as a scaffold for assembling macromolecular complexes which includes enzymes (PLC gamma, Vav and Btk) and additional linker proteins (Grb2 and Nck) (1-3) . In addition, BLNK binds Btk and is required for activation of the transcription factor NF- B. Tyrosine phosphorylation of BLNK by Syk provides specific docking sites for these SH2-containing effector proteins which in turn permits phosphorylation and/or activation of their respective signaling pathways. Applications: Suitable for use in Western Blotting, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blotting: 1:500 Immunohistochemistry: 1:50 Immunoprecipitation: 1:20 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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