Technical Data
B Cell Linker Protein, phosphorylated (Tyr96) (BLNK, BASH, BLNK-s, Ly57, MGC111051, SLP-65, SLP65)
BLNK (B cell linker protein) is an adaptor molecule that plays key roles from B cell activation through B cell antigen receptor (BCR) engagent. BLNK acts on the inferface between BCR-associated Syk and the downstream signaling cascade (1,2). BLNK has multiple SH2 binding motifs (YXXP) at its N-terminus and an SH2 domain at its C-terminus. After BCR ligation, BLNK is phosphorylated by Syk on its multiple YXXP motifs including Tyr72, Tyr84, Tyr96 and Tyr178 (1). The phosphorylation on these motifs provides docking sites for signaling molecules such as Btk, PLCgamma and Vav. They bind to BLNK through their SH2 domains and cooperatively activate downstream signaling (3,4). BLNK can also interact with phosphorylated targets such as HPK1 through its SH2 domain to activate the pathway (5).

Suitable for use in Western Blotting, Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunoprecipitation: 1:100

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
Synthetic phosphopeptide (KLH coupled) corresponding to residues around Tyr96 of human BLNK.
Purified by protein A and peptide affinity chromatography.
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Detects endogenous levels of Tyr96 phosphorylated BLNK.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Kurosaki, T. and Tsukada, S. (2000) Immunity 12, 15. (2) Fu, C. et al. (1998) Immunity 9, 93103. (3) Ishiai, M. et al. (1999) Immunity 10, 117125. (4) Baba, Y. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 25822586. (5) Tsuji, S. et al. (2001) J. Exp. Med. 194, 529539.