Technical Data
B0807-06K
Bcl-2 (B Cell Leukemia 2)
Description:
Bcl-2 (B-cell lymphoma protein 2 alpha) is an anti-apoptotic protein located primarily in the outer mitochondrial membrane. It blocks the apoptotic death of some cells such as lymphocytes. BCL-2 is thought to regulate cell death by controlling the mitochondrial membrane permeability during apoptosis. Bcl-2 protein has anti-apoptotic functions through inhibiting caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:250 - 500
Starting dilution: 1:500
Optimal dilutions to be determined by the researcher.

Hybridoma:
F0 myeloma cells with spleen cells from BALB/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b11H1Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant corresponding to aa1-211 from human Bcl-2 expressed in E. coli.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes human Bci-2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zamzami N., et al,. (1998) Oncogene. 16(17):2265-82. 2. Fernandez-Sarabia MJ., et al,. (1993) Nature. 366(6452):274-5.