Technical Data
B0807-07BX
Bcl 2a (B Cell Leukemia 2a) (BSA & Azide Free)
Description:
Expression of bcl-2a oncoprotein has been shown to inhibit the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2a protein, whereas the normal or hyperplastic germinal centers are negative. Bcl-2 belongs to a family of genetically related proteins that appear to regulate apoptosis or programmed cell death. Bcl-2 is encoded by a proto-oncogene (bcl-2) that is the mammalian homolog of ced-9, the C. elegans death suppressor gene. Overexpression of Bcl-2 appears to suppress apoptosis in a number of mammalian experimental systems. Recent evidence indicates that Bcl-2 binds to other family members and R-ras and influences antioxidant pathways in the cell. Bcl-2 is a 25kDa protein that has extensive amino acid homology with Bax. It forms homodimers or heterodimers with Bax and the apoptotic activity depends on the balance of both molecules. The t(14;18) translocation, which is a hallmark of human follicular lymphoma, generally involves the immunoglobulin heavy chain gene on chromosome 14 and the bcl-2 gene on chromosome 18. This translocation results in overexpression of the bcl-2 gene product (Bcl-2), promoting cell survival by blocking programmed cell death induced by different apoptotic stimuli.
Applications:
Suitable for use in Flow Cytometry, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1ug/ml for 2hrs at RT.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min, followed by cooling at RT for 20 min.
Optimal dilution determined by the researcher.

Positive Control:
Tonsil or follicular lymphomas. Raji or HeLa cells.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10.N.24Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa41-54 Cys-GAAPAPGIFSSQPG of human Bcl 2a. Cellular Localization: Cytoplasm MW of antigen: 25-26kD.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See B0807-07B.
Specificity:
Recognizes human Bcl-2a at ~25kD. Species Crossreactivity: monkey. Does not react with mouse or rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wolter KG, et. al. Journal of Cell Biology, 1997, 139(5):1281-92.