Technical Data
B0807-07BX
Bcl 2a (B Cell Leukemia 2a) (BSA & Azide Free)
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Technical Data |
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B0807-07BX |
Bcl 2a (B Cell Leukemia 2a) (BSA & Azide Free) |
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Description: Expression of bcl-2a oncoprotein has been shown to inhibit the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2a protein, whereas the normal or hyperplastic germinal centers are negative. Bcl-2 belongs to a family of genetically related proteins that appear to regulate apoptosis or programmed cell death. Bcl-2 is encoded by a proto-oncogene (bcl-2) that is the mammalian homolog of ced-9, the C. elegans death suppressor gene. Overexpression of Bcl-2 appears to suppress apoptosis in a number of mammalian experimental systems. Recent evidence indicates that Bcl-2 binds to other family members and R-ras and influences antioxidant pathways in the cell. Bcl-2 is a 25kDa protein that has extensive amino acid homology with Bax. It forms homodimers or heterodimers with Bax and the apoptotic activity depends on the balance of both molecules. The t(14;18) translocation, which is a hallmark of human follicular lymphoma, generally involves the immunoglobulin heavy chain gene on chromosome 14 and the bcl-2 gene on chromosome 18. This translocation results in overexpression of the bcl-2 gene product (Bcl-2), promoting cell survival by blocking programmed cell death induced by different apoptotic stimuli. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min, followed by cooling at RT for 20 min. Optimal dilution determined by the researcher. Positive Control: Tonsil or follicular lymphomas. Raji or HeLa cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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