Technical Data
Bcl 2 Modi ng Factor, CT, Blocking Peptide (Bmf)
Molecular Biology Storage: -20CShipping: Blue Ice
Synthetic peptide (ALNGEENRNGAGPR) corresponding to amino acids 171 to 184 of human Bmf (1).

Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identi ed in human and mouse and designated Bmf (for Bcl-2-modi ng factor) (1). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cells actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin laments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program (1,2). Bmf is constitutively expressed in many tissues (1,2).

Applications: Suitable for use in ELISA and Western Blot. Other applications not tested.

Storage and Stability: Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile 25% glycerol and store at -20C. May be stored at 4C for short-term only. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Synthetic peptide
Purity: Purified 60-70%
Concentration: ~0.2mg/ml
Form: Supplied as a lyophilized powder.

Intended for research use only, not for use in human, therapeutic or diagnostic applications.
1. Puthalakath H, Villunger A, OReilly LA, Beaumont JG, Coultas L, Cheney RE, Huang DC, Strasser A. Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis. Science. 2001;293(5536):182932.
2. Hunt A, Evan G. Apoptosis. Till death us do part. Science. 2001;293(5536):17845.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.