		Technical Data
B0807-12L	BCL10 (B-cell CLL, B-cell Lymphoma/Leukemia 10, CARD-containing molecule enhancing NF-kappa-B, CARD-like apoptotic protein, CARMEN, c-E10, CED-3/ICH-1 prodomain homologous E10-like regulator, Cellular-E10, Cellular homolog of vCARMEN, CIPER, CLAP, hCLAP,

Description:
Bcl-10 was originally identified through its direct involvement in t(1;14)(p22;q32) of mucosa-associated lymphoid tissue (MALT) lymphoma. The Bcl-10 molecule is predominantly located in the cytoplasm but expression has also been reported in the nucleus of some lymphomas. Recent reports suggest that Bcl-10 is essential for nuclear factor (NF)-kappaB activation after lymphocyte receptor antigen stimulation and for the development of mature B-cells.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat 10ul labels 1x10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.

Recommended Negative Controls
Mouse IgG1 Negative Control:RPE
Mouse IgG1 Negative Control:RPE

Storage and Stability:
Lyophilized powder may be stored at 4°C before opening. DO NOT FREEZE! Reconstitute with ddH2O. Aliquot and store at 4ºC. Stable at 4°C as an undiluted liquid for 6 months. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.

Type	Isotype	Clone	Grade
Mab	IgG1	9E23	Affinity Purified

Size		Storage	Shipping	Source	Host
100 Tests		4°C Do Not Freeze	Blue Ice	Human	Mouse

Concentration:

~100 tests/ml

Immunogen:

Full length recombinant Bcl-10.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a lyophilized powder in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.

Specificity:

Recognizes human B-cell lymphoma/leukaemia 10 (Bcl-10).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Ye, H. et al. (2005) MALT lymphoma with t(14;18)(q32;q21)/IGH-MALT1 is characterized by strong cytoplasmic MALT1 and BCL10 expression. J Pathol. 205:293 – 301.