Technical Data
B0899-03N
BD1 (Beta-defensin 1, BD-1, hBD-1, Defensin, beta 1, DEFB1, BD1, HBD1)
Description:
Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely beta-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms.

The genes encoding human alpha and beta defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated and based on distinctive although similar, tri-disulfide linkages in the peptides. beta-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two defensins, HBD-1 and HBD-2, have been identified to date. Rat RBD-1 and RBD-2) and mouse (MBD1-4) homologues of the human beta-defensin have also been identified. alpha-defensins are encoded by genes designated DEFA1-6, whereas human bdefensins are encoded by the DEFB1 and DEFb2 genes.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:50,000
Western Blot: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to 17aa of human HBD-1 (KLH).
Purity:
Serum
Form
Supplied as a lyophilized powder, 0.05% sodium azide.
Specificity:
Recognizes human BD1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Huttner Km (1997) FEBS Lett. 413, 45-69. 2. Liu L (1997) Genomics 43, 316-320. 3. McCray PB (1997) Am, J. Respir. Cell. Mol. Biol. 16, 343-349. 4. Bensch KW (1995 FEBS Lett. 368, 331-335. 5. Bartels J (1997) Nature 387, 861. 6. Ganz T (1999) Science 286, 420. 7. Yang D (1999) Science 286, 525.