Technical Data
BD1, Control Peptide (Beta-defensin 1, BD-1, hBD-1, Defensin, beta 1, DEFB1, BD1, HBD1)
Molecular Biology Storage: -20CShipping: Blue Ice
Control peptide for B0899-03N and B0899-03P.

Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely beta-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms.

The genes encoding human alpha and beta defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated and based on distinctive although similar, tri-disulfide linkages in the peptides. beta-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two defensins, HBD-1 and HBD-2, have been identified to date. Rat RBD-1 and RBD-2) and mouse (MBD1-4) homologues of the human beta-defensin have also been identified. alpha-defensins are encoded by genes designated DEFA1-6, whereas human bdefensins are encoded by the DEFB1 and DEFb2 genes.

Synthetic peptide corresponding to 17aa of human HBD-1, near the N-terminus.

Molecular Weight:

Suitable for use in ELISA and Antibody Blocking. It is not suitable for use in Western. Other applications not tested.

Recommended Dilution:
ELISA: 1ug/ml and detected with antibodies
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Synthetic peptide
Purity: Purified
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder from PBS, pH7.5.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Huttner Km (1997) FEBS Lett. 413, 45-69. 2. Liu L (1997) Genomics 43, 316-320. 3. McCray PB (1997) Am, J. Respir. Cell. Mol. Biol. 16, 343-349. 4. Bensch KW (1995 FEBS Lett. 368, 331-335. 5. Bartels J (1997) Nature 387, 861. 6. Ganz T (1999) Science 286, 420. 7. Yang D (1999) Science 286, 525.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.