Technical Data
B0899-03R
BD1 (Beta-defensin 1, BD-1, hBD-1, Defensin, beta 1, DEFB1, BD1, HBD1)
Description:
Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely beta-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms.

The genes encoding human and -defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated - and - based on distinctive, although similar, tri-disulfide linkages in the peptides. beta-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six-defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two-defensins, HBD-1 and HBD-2, have been identified to date. Rat (RBD-1 and RBD-2) and mouse (MBD1-4) homologues of the human beta-defensin have also been identified. alpha-defensins are encoded by genes designated DEFA1-6, whereas human beta-defensins are encoded by the DEFB1 and DEFb2 genes.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1ul/ml
Western Blot: 1-3ug/ml (ECL), using native or denatured protein.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Recombinant corresponding to human Beta-Defensin 1, full length (Accession #P60022).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.1% BSA.
Specificity:
Recognizes human BD-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1..Huttner Km (1997) FEBS Lett. 413, 45-69. 2. Liu L (1997) Genomics 43, 316-320. 3. McCray PB (1997) Am, J. Respir. Cell. Mol. Biol. 16, 343-349. 4. Bensch KW (1995) FEBS Lett. 368, 331-335. 5. Bartels J (1997) Nature 387, 861. 6. Ganz T (1999) Science 286, 420. 7. Yang D (1999) Science 286, 525.