Technical Data
B0900-25
BD1 (beta Defensin-1)
Description:
Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35 aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms. The genes encoding human alpha and beta-defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated alpha and beta based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six a-defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two b-defensins, HBD-1 and HBD-2, have been identified.

Alpha-defensins are encoded by genes designated DEFA1-6, whereas human b-defensins are encoded by the DEFB1 and DEFb2 genes. HD1-4 are expressed in neutrophils, whereas HD5 and HD6 are expressed in epithelial cells of the intestinal and reproductive tract. HD1-3/HNP1-3 make up about 30% of the neutrophil's total granule protein. The HNPs are 29 to 30 amino acids long and are identical in sequence except at the N-terminal amino acid. DEFA1 and DEFA3 differ from each other only by their terminal ala and asp, respectively. DEFA2 is identical to both except that it has 29 instead of 30 amino acids.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000
ELISA: 1:10,000-50,000.
Optimal dilutions to be determined by the researcher.

Control Peptide: B0900-20

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide (17aa) near the N-terminus of mature human defensin-1 (KLH coupled).
Purity:
Serum.
Form
Supplied as a liquid, neat serum, 0.05% sodium azide, 40% glycerol.
Specificity:
Recognizes BD1. No significant homology exists with other beta-defensins (2-4). Species Sequence Homology: Monkey beta deffensin-1: 94%. No significant homology exists with mouse or rat defensin-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Huttner, K.M., et al., FEBS Lett. 413: 45-69 (1997). 2. Liu, L., et al., Genomics 43: 316-320 (1997). 3. McCray, P.B., et al., Am. J. Respir. Cell. Mol. Biol. 16: 343-349 (1997). 4. Bensch, K.W., et al., FEBS Lett. 368: 331-335 (1995). 5. Bartels, J., et al., Nature 387: 861 (1997). 6. Ganz, T., Science 286: 420 (1999). 7. Yang, D., et al., Science 286: 525 (1999).