Technical Data
B0900-30
BD1 (beta Defensin-1)
Description:
Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides. They were originally identified in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-Sheet structure. Defensins are classified into two families designated alpha and beta based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. Alpha-defensins are encoded by genes designated DEFA1-6. Human b-defensins are encoded by the DEFB1 and DEFb2 genes. HD1-4 are expressed in neutrophils. HD5 and HD6 are expressed in epithelial cells of the intestinal and reproductive tract. HD1-3/HNP1-3 make up about 30% of the neutrophil's total granule protein. The HNPs are 29-30aa long and are identical in sequence except at the N-terminal amino acid. DEFA1 and DEFA3 differ from each other only by their terminal ala and asp, respectively. DEFA2 is identical to both except that it has 29 instead of 30 amino acids. Human defensin-1 is produced from 68aa precursor (mature peptide 33-69aa) peptide. Mouse defensin-1 is produced from 69aa precursor (mature peptide 33-69aa).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml using ECL.
ELISA: 0.5-1ug/ml, control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Control Peptide: B0900-20

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide (17aa) near the N-terminus of mature human defensin-1 (KLH coupled).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA, 40% glycerol.
Specificity:
Recognizes human BD1. No significant homology exists with mouse or rat defensin-1 or other beta-defensins (2-4). Species Sequence Homology: Monkey beta deffensin-1: 94%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Huttner, K.M., et al., FEBS Lett. 413:45-69 (1997). 2. Liu, L., et al., Genomics 43:316-320 (1997). 3. McCray, P.B., et al., Am. J. Respir. Cell. Mol. Biol. 16:343-349 (1997). 4. Bensch, K.W., et al., FEBS Lett. 368:331-335 (1995). 5. Bartels, J., et al., Nature 387:861 (1997). 6. Ganz, T., Science 286:420 (1999). 7. Yang, D., et al., Science 286:525 (1999). 8. Wase KB, 2004: Microbes and Infrction 6, 51-57, IHC 9. Supp DM 2004: Burns 30, 643-649, IHC.