Technical Data
B0901-03B
BD2 (beta Defensin-2, beta 2 Defensin, Defensin beta 2, BD 2, BD-2, DEFB2, DEFB-2, HBD 2, HBD2, HBD-2, DEFB102, Skin Antimicrobial Peptide 1, SAP1)
Description:
Expression of BD-2 is induced during inflammation in response to bacterial products and cytokines, and is initially expressed in a precursor form, which is cleaved to release the C-terminal active portion of the protein.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.5ug/ml
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C for short-term only.Stable for 12 months at -20C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
Not determined
Immunogen:
Recombinant human Defensin beta 2.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4. No preservative added. Reconstitute with 500ul sterile 40-50% glycerol, ddH2O.
Specificity:
Recognizes human Defensin beta-2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bals, R. et al. (1998) Human beta-Defensin 2 is a salt-sensitive peptide antibiotic expressed in human lung. J. Clin. Invest. 102: 874-880. 2. Harder, J. et al. (2000) Mucoid Pseudomonas aeruginosa, TNF-a, and IL-1beta, but not IL-6, induce human beta-Defensin-2 in respiratory epithelia. Am. J. Respir. Cell Mol. Biol. 22: 714-721. 3. Harder, J. et al. (2000) Mucoid Pseudomonas aeruginosa, TNF-a, and IL-1beta, but not IL-6, induce human beta-Defensin-2 in respiratory epithelia. Am. J. Respir. Cell Mol. Biol. 22: 714-721.