		Technical Data
B0901-03B1	BD2 (beta Defensin-2, beta 2 Defensin, Defensin beta 2, BD 2, BD-2, DEFB2, DEFB-2, HBD 2, HBD2, HBD-2, DEFB102, Skin Antimicrobial Peptide 1, SAP1) (Biotin)

Description:
Expression of BD-2 is induced during inflammation in response to bacterial products and cytokines, and is initially expressed in a precursor form, which is cleaved to release the C-terminal active portion of the protein.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1ug/ml
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.

Recommended Pair:
Suitable for use as the detection or conjugate (label) antibody in sandwich immunoassay. Matched to B0901-03B as the capture or solid phase antibody.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.1mg/ml (after reconstitution)

Immunogen:

Recombinant human Defensin beta 2.

Purity:

Purified by affinity chromatography.

Form

Supplied as a lyophilized powder from PBS. No preservative added. Labeled with Biotin. Reconstitute with 500ul of sterile PBS, 0.1% BSA.

Specificity:

Recognizes human Defensin beta-2, a 4.3kD cationic antimicrobial peptide expressed primarily by epithelial cells of the respiratory tract and skin, and an important component of the innate immune response against microbial infections.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Bals, R. et al. (1998) Human beta-Defensin 2 is a salt-sensitive peptide antibiotic expressed in human lung. J. Clin. Invest. 102: 874-880. 2. Harder, J. et al. (2000) Mucoid Pseudomonas aeruginosa, TNF-a, and IL-1beta, but not IL-6, induce human beta-Defensin-2 in respiratory epithelia. Am. J. Respir. Cell Mol. Biol. 22: 714-721. 3. Harder, J. et al. (2000) Mucoid Pseudomonas aeruginosa, TNF-a, and IL-1beta, but not IL-6, induce human beta-Defensin-2 in respiratory epithelia. Am. J. Respir. Cell Mol. Biol. 22: 714-721.