Technical Data
B0901-05
BD2, Human, Mouse, Rat, Control Peptide (beta Defensin-2)
50ug
Molecular Biology Storage: -20CShipping: Blue Ice
Control Peptide for B0901-08 (Serum) and B0901-13 (Affinity purified antibody).

Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides. They were originally identified in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules. They are released into phagolysosomes where they contribute to the killing of engulfed microorganisms. The genes encoding human a- and b-defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated a- and b- based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six a b-defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two b-defensins, HBD-1 and HBD-2, have been identified to date. Rat (RBD-1 and RBD-2) and mouse (MBD1-4) homologues of the human beta-defensin have also been identified.

Antigen:
Three different synthetic peptides (KLH coupled): Human (14aa from human defensin-2), rat (14aa from rat defensin-2) and mouse (17aa from mouse defensin-2). Corresponding to the N-terminus of mature beta-defensin-2. No significant sequence homology with other defensins.

Applications:
Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 50-100ng of control peptide/well
Antibody Blocking: 5-10ug per 1ul B0901-08 or per 1ug B0901-13.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Human, mouse & rat synthetic peptides
Purity: Purified
Concentration: ~1mg/ml
Form: Supplied as a liquid in PBS, pH 7.5, 0.05% sodium azide.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Harder, J., et al., Nature 387: 861 (1997). 2. Diamond, J., et al., Infect. Immun. 68: 113-119 (2000). 3. Morrision, G.M., et al., FEBS Lett. 442: 112-116 (1999). 4. Liu, L., et al., Gene 222: 237-244 (1998). 5. Bartels, J., et al., Nature 387: 861 (1997). 6. Ganz, T., Science 286: 420 (1999). 7. Yang, D., et al., Science 286: 525 (1999).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.