Technical Data
B0901-08
BD2 (beta Defensin-2)
Description:
Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides. They were originally identified in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules. They are released into phagolysosomes where they contribute to the killing of engulfed microorganisms. The genes encoding human a- and b-defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated a- and b- based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six a b-defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two b-defensins, HBD-1 and HBD-2, have been identified to date. Rat (RBD-1 and RBD-2) and mouse (MBD1-4) homologues of the human beta-defensin have also been identified.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000 (ECL)
ELISA: 1:10,000-1:50,000. Use 1ug/ml of B0901-05 Control Peptide to coat ELISA plates.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceRabbit
Concentration:
Not Determined
Immunogen:
Three different synthetic peptides corresponding to human (14aa from human BD2), rat (14aa from rat defensin-2) and mouse (17aa from mouse BD2) (KLH). Epitope: N-terminus of mature beta-defensin-2 conjugated to KLH.
Purity:
Serum
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes human beta defensin-2. No significant sequence homology with other defensins. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Harder, J., et al., Nature 387: 861 (1997). 2. Diamond, J., et al., Infect. Immun. 68: 113-119 (2000). 3. Morrision, G.M., et al., FEBS lett. 442: 112-116 (1999). 4. Liu, L., et al., Gene 222: 237-244 (1998). 5. Bartels, J., et al., Nature 387: 861 (1997). 6. Ganz, T., Science 286: 420 (1999). 7. Yang, D., et al., Science 286: 525 (1999).