Technical Data
B0901-15
BD2, Recombinant, Human (beta Defensin-2)
10ug
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Technical Data |
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B0901-15 |
BD2, Recombinant, Human (beta Defensin-2) |
10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Human defensin-2 (hBD-2; 41 aa): Mol wt. 4.3kD GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P HBD-2 was expressed in E. coli, refolded, purified to >98% purity. Endotoxin content is insignificant (<0.1ng/ug (1.0 EU/ug). Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35 aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms. The genes encoding human a and ß -defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated a-and ß-based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six a -defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two ß -defensins, HBD-1 and HBD-2, have been identified to date. Rat (RBD-1 and RBD-2) and mouse (MBD1-4) homologues of the human beta-defensin have also been identified. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. CAS Number: N/A Molecular Weight: 4.3kD |
Source: Human Purity: 98% Concentration: ~1mg/ml Form: Supplied as a liquid in10 mM acetic acid. Specificity: The HBD-2 recombinant protein activity was determined by its ability to chemoattract immature dendritic cells using a concentration of 1-10ng/ml Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1.Harder J et al (1997) Nature 387, 861. 2. Diamond J. et al (2000) Infect. Immun. 68, 113-119. 3. Morrision GM et al (1999) FEBS lett. 442, 112-116. 4. Liu L et al (1998) Gene 222, 237-244. 5. Bartels J et al (1997) Nature 387, 861. 6. Ganz T (1999) Science 286, 420. 7. Yang D et al (1999) Science 286, 525.
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