Technical Data
B0902
BD3 (beta Defensin-3)
Description:
Beta Defensin-3 is a novel 5-9kD nonhemolytic antimicrobial protein peptide which may be important in the innate epithelial defense of infections by various microorganisms seen in skin and lung (such as cystic fibrosis).

Applications:
Suitable for use Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot:
Tricine-SDS-PAGE gels: 1:500-1:1000. Migrates as a 5-6kD band.
Tricine-SDS-urea PAGE gels: Native Beta Defensin 3 migrates as a 9kD band. The larger size is because of the reduction of the three cysteine bridges present in the peptide protein.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide consisting of aa 23-33 (GIINTLQKYYC) of the human beta-defensin-3 protein
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes the 5kD human Defensin-3, beta protein. Species Crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.